Scientific Publications and Presentations

MicroRNA-375 Suppresses the Growth and Invasion of Fibrolamellar Carcinoma

February 11, 2019

Dinh, Jewell, ... & Sethupathy
Journal article published in Cellular and Molecular Gastroenterology and Hepatology

While the significance of the DNAJB1-PRKACA fusion gene in fibrolamellar carcinoma has been established and validated, the role of microRNAs (miRNAs) in FLC remains unclear. MicroRNAs have been implicated as drivers in many diseases including a variety of cancers. This study analyzed small RNA sequencing data to identify dysregulated miRNAs in FLC tumors and identified miR-375 as the most dysregulated miRNA. The study showed that over-expression of miR-375 mitigated the growth and invasive potential of FLC tumor cells. The study analyzed and validated the findings using a patient-derived xenograft model, CRISPR-Cas9 transfer of the DNAJB1-PRKACA fusion gene into mice, and a new FLC cell line.

Implications: These findings suggest a potentially new therapeutic approach for the disease. Further studies are needed to determine specifically how DNAJB1-PRKACA suppresses miR-375 expression and whether miR-375 has additional important targets in FLC.

The complete article can be read below:

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